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2-chloroethanol Degradation by wild type and mutants

Time:2015/12/3 7:03:15

Contact sensitivity to 1-(4-(2-chloroethyl)phenyl)-2-chloroethanol in a polymer chemist
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Degradation of 2-chloroethanol by wild type and mutants of Pseudomonas putida US2
A strain of Pseudomonas putida was isolated that was able to degrade 2-chloroethanol. The degradation proceeded via 2-chloroacetaldehyde and chloroacetate to glycolate. In crude extracts the enzymes for this degradation pathway could be detected. All enzymes proved to be inducible. The dehalogenase that catalyzed the dehalogenation of chloroacetate to glycolate was further characterized. It consisted of a single polypeptide chain with a molecular mass of 28 kDa. After induction the dehalogenase was expressed at a high level. In a mutant resistant to high concentrations of 2-chloroethanol the dehalogenase was no longer expressed. The mechanism of resistance seemed to be due to the inability to convert chloroacetate and export of this compound out of the cell.