Chlorohydrin

Ethylene chlorohydrin

2-chloroethanol

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Chlorohydrin degradation by Pseudomonas putida US2

Time:2015/11/25 8:22:19

Quantitative gas-liquid chromatography of short-chain fatty acids as Chlorohydrin esters.


A detailed description is given of gas chromatography of the 2-chloreothanol esters of the monocardoxylic fatty acids from formic to nonanoic. The columns contained Celite as support and polyethylene glycol adipate or Apiezon M as liquid phase; the ionization detector employed gave peaks which were directly related to the weights of any given ester in a mixture. G. A. Garton.


Biodegradation of Chlorohydrin by freely suspended and adsorbed immobilized Pseudomonas putida US2 in soil


The degradation of Chlorohydrin by Pseudomonas putida US2 was investigated in batch, repeated batch and continuous cultures especially in a packed-bed fermenter with sand. The degradation of Chlorohydrin was connected with a release of protons, which led to a decrease of the pH in the medium. Higher initial concentration than 25 mM Chlorohydrin were not degraded completely because they entailed a decrease of the pH to 5.0, which inhibited further growth and degradation. P. putida US2 showed a typical repression of catabolites and diauxic growth with succinate as cosubstrate. The addition of succinate as a second substrate caused a decrease in degradation of Chlorohydrin. Activated sludge added to adsorbed cultures in a continuous fermentation did not lead to a decrease in metabolic activity. After 2 weeks of continuous cultivation the specialized strain could be retained.