Chlorohydrins in protein hydrolysates?

Chlorohydrins in protein hydrolysates

Four samples of neutral fractions of protein hydrolysates were separated by gas chromatography and the individual components were identified from the mass spectra obtained. Some of the identified compounds were not previously reported as volatile components of foodstuffs. Three of these compounds namely 3-chloropropan-1-ol, 2,3-dichloropropan-1-ol, and 1,3-dichloropropan-2-ol, were toxic chlorohydrins. 1,3-Dichloropropan-2-ol was present in samples in concentrations 0.17 to 0.94 mg/kg. To check the possibilities of the formation of these chlorohydrins and to find their precursor, glycerol, and hydrochloric acid mixtures were heated under conditions of producing protein hydrolysates. All three chlorohydrins, formerly found in protein hydrolysates have been identified.

A New Palladium(II)-Catalyzed Asymmetric Chlorohydrin Synthesis

A new approach is reported for secondary-ion time-of-flight mass spectrometry (TOF-SIMS) sample preparation. The method involves the use of halide additives or halide modification of silver substrate surfaces to promote analyte cationization and protonation. The enhancement of signal intensity has been demonstrated for neutral organic lipophilic and hydrophilic compounds including various small peptides and nucleosides. Improvement factors range from 2-30 for cationized species to 20-2000 for protonated species. However, the new sample preparation does not affect the signal intensities of preformed ionic species. The sample preparation approach is applicable to a wide variety of neutral compounds and should find broad use for organic analysis by TOF-SIMS. (C) 1998 John Wiley & Sons, Ltd.