ethylene chlorohydrin in fumigated foodstuffs

High Performance Liquid Chromatography Determination of Ethylene Glycol and Ethylene Chlorohydrin in Tissues

A simultaneous determination of ethylene glycol (EG) and ethylene chlorohydrin (EC), which are formed during sterilization of bone and dura mater with ethylene oxide, has been reported. The EG and EC residues in tissues were extracted with HPLC grade water and analyzed by reversed-phase high-performance liquid chromatography (HPLC) with a RefractoMonitor detector. The isocratic elution was employed with HPLC grade water as the solvent. Since the reference chamber of the detector was filled with solvent and was not changed during the run, the use of gradient programming was not employed. With spherisorb ODS (5 08;m) C18 column and 20 08;L sample injection, the retention times for EG and EC were (104±2) sec and (202±4) sec, respectively. The unknown concentration was calculated from the calibration curve and the weight of the sample tissue. The HPLC analysis provided a simple, accurate and reproducible method of determination of EG and EC residues in ethylene-oxide sterilized tissues.

Determination of residual ethylene chlorohydrin (ECH) in fumigated foodstuffs by glass capillary gas chromatography

A simple quantitative method for the determination of residual ethylene chlorohydrin in fumigated foodstuffs is described. The method is based on a solvent extraction and quantitative determination of ethylene chlorohydrin by capillary gas chromatography using dibutyl ketone as internal standard. The chromatographic identification of ethylene chlorohydrin is performed on a glass capillary column coated with Carbowax 20 M. The detection limit of this analytical system is 2 ng. Recoveries of added ethylene chlorohydrin from foodstuffs are between 85% and 99% in the concentration range of up to 100 ppm. Of a total of 340 samples analysed 11,6% were positive for ethylene chlorohydrin.